Our Claro product range

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Inks for extrusion-based bioprinting

Many of the obstacles in bioprinting are related to the use of biomaterials that are low in cell-compatibility and/or with no stable flow behavior. Derived from natural collagen, gelatin contains essential natural amino acid combinations (such as arginine-glycine-aspartic acid) and metalloproteinase (MMP)-sensitive degradation sequences that are responsible for promoting cell adhesion and matrix remodeling. Gelatin is also known for its non-antigenicity and non-immunogenic properties which represent an advantage over collagen (Davidenko et al., 2016). Although highly cell-compatible, gelatin-based bioinks are extremely sensitive to the thermal environment and thermal history of the formulation during printing - even after the addition of crosslinking agents or chemical modifications of the gelatin backbone structure.

In most of the extrusion-based bioprinters, the nozzle is connected to a syringe with a luer-lock system which is not heated by the heating element. As a result, uncontrollable gelation of regular gelatin starts to  take place even if the printing temperature is set above the gelation temperature that is in the range of 20 to 25 °C. Gel pieces deposited on the internal surface of the nozzle impede uniform flow causing imperfections in the construct. Ultimately, the nozzle becomes clogged.

ClaroBGI800

ClaroBGI800 is gelatin-based biomaterial that combines all the advantages of gelatin methacryloyl (GelMA) with previously unseen levels of flow for bioprinting. The rheological profile of ClaroBGI800 allows bioprinting at ambient conditions (21 ± 1°C) without the requirement to control the temperature of the print-head. ClaroBGI800 is crosslinkable under UV light in the presence of a photo initiator (e.g. Irgacure 2959 or LAP). ClaroBGI800 is available from low to high degree of functionalization (DoF), ensuring a flexible and stable matrix for cell culturing.​

Our proprietary technology brings a wider formulation window for bioinks in extrusion based systems by overcoming nozzle clogging, and poor shape-fidelity observed with regular GELMA products. In addition, ClaroBGI800 is produced under high quality standards of purification, ensuring ultra-low levels of impurities such as methacrylic acid​ and guaranteeing batch to batch consistency.

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Disclaimer:

ClaroBGI800 is for research purposes only, not for in vivo use in humans or animals. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Download SDS here

REFERENCES

Davidenko, N., Schuster, C. F., Bax, D. V., Farndale, R. W., Hamaia, S., Best, S. M., & Cameron, R. E. (2016). Evaluation of cell binding to collagen and gelatin: a study of the effect of 2D and 3D architecture and surface chemistry. Journal of Materials Science: Materials in Medicine, 27(10). https://doi.org/10.1007/s10856-016-5763-9